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rat igg2b  (Bio X Cell)


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    Structured Review

    Bio X Cell rat igg2b
    The effects of PD-L1 blockade on airway hyperresponsiveness, inflammatory cell infiltration, and the expression of <t>immunoglobulin</t> in lung tissues of asthmatic mice were investigated. (A) Hyperresponsive changes in the airways of mice detected after stimulation with different concentrations of acetylcholine. (B) Total cells in BALF. (C) Number of eosinophils in BALF. (D–E) The expression levels of OVA-IgE and <t>IgG1</t> were measured in BALF. PD-L1, Programmed cell death ligand 1. OVA-IgE, Ovalbumin-specific Immunoglobulin E. <t>OVA-IgG1,</t> Ovalbumin-specific Immunoglobulin G1. Data were presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
    Rat Igg2b, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isotype+control+igg2b/InVivoMAb+rat+IgG2b+isotype+control%2C+anti-keyhole+limpet+hemocyanin/pmc13634089-42-27-36
    Average 97 stars, based on 1248 article reviews
    rat igg2b - by Bioz Stars, 2026-10
    97/100 stars

    Images

    1) Product Images from "Anti-PD-L1 improves barrier function and reduces CD103 + DC cell accumulation by regulating gut and lung microbiota and its metabolites to alleviate asthma in juvenile mice"

    Article Title: Anti-PD-L1 improves barrier function and reduces CD103 + DC cell accumulation by regulating gut and lung microbiota and its metabolites to alleviate asthma in juvenile mice

    Journal: Biomedical Journal

    doi: 10.1016/j.bj.2025.100944

    The effects of PD-L1 blockade on airway hyperresponsiveness, inflammatory cell infiltration, and the expression of immunoglobulin in lung tissues of asthmatic mice were investigated. (A) Hyperresponsive changes in the airways of mice detected after stimulation with different concentrations of acetylcholine. (B) Total cells in BALF. (C) Number of eosinophils in BALF. (D–E) The expression levels of OVA-IgE and IgG1 were measured in BALF. PD-L1, Programmed cell death ligand 1. OVA-IgE, Ovalbumin-specific Immunoglobulin E. OVA-IgG1, Ovalbumin-specific Immunoglobulin G1. Data were presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: The effects of PD-L1 blockade on airway hyperresponsiveness, inflammatory cell infiltration, and the expression of immunoglobulin in lung tissues of asthmatic mice were investigated. (A) Hyperresponsive changes in the airways of mice detected after stimulation with different concentrations of acetylcholine. (B) Total cells in BALF. (C) Number of eosinophils in BALF. (D–E) The expression levels of OVA-IgE and IgG1 were measured in BALF. PD-L1, Programmed cell death ligand 1. OVA-IgE, Ovalbumin-specific Immunoglobulin E. OVA-IgG1, Ovalbumin-specific Immunoglobulin G1. Data were presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Techniques Used: Expressing

    Related Articles

    Injection:

    Article Title: Chemotherapy-driven intestinal dysbiosis and indole-3-propionic acid rewire myelopoiesis to promote a metastasis-refractory state.
    Article Snippet: Mice were gavage daily with IPA (Sigma # 57400-5G) at 20mg/kg diluted in sterile PBS, as described35. .. To deplete CD4+ T cells, mice were i.p. injected with 10mg/kg of anti-CD4 (Bioxcell; ##BE00031) or 10mg/kg isotype control IgG2b (BioXcell #BE0090) at day 3, 5 and 8 after hepatic metastases implantation. .. To deplete CD4+ T cells, mice were i.p. injected with 10mg/kg of anti-CD4 (Bioxcell; ##BE00031) or 10mg/kg isotype control IgG2b (BioXcell #BE0090) at day 3, 5 and 8 after hepatic metastases implantation.

    Article Title: Chemotherapy-driven intestinal dysbiosis and indole-3-propionic acid rewire myelopoiesis to promote a metastasis-refractory state
    Article Snippet: .. To deplete CD4 + T cells, mice were i.p. injected with 10 mg/kg of anti-CD4 (Bioxcell; ##BE0003-1) or 10 mg/kg isotype control IgG2b (BioXcell #BE0090) at day 3, 5 and 8 after hepatic metastases implantation. ..

    Article Title: Induction of senescence renders cancer cells highly immunogenic
    Article Snippet: .. For depletion of immune populations, animals received 100 μg of blocking antibodies (anti-CD4 (clone GK1.5, BioxCell), anti-CD8 (clone 2.43, BioxCell), anti-CD11b (clone M1/70, BioxCell)) or isotype control IgG2b (clone LTF-2, BioxCell) via intraperitoneal injection of a total volume of 100 μL on days -1, 3, and 8 of the immunization experiments. ..

    Control:

    Article Title: Chemotherapy-driven intestinal dysbiosis and indole-3-propionic acid rewire myelopoiesis to promote a metastasis-refractory state.
    Article Snippet: Mice were gavage daily with IPA (Sigma # 57400-5G) at 20mg/kg diluted in sterile PBS, as described35. .. To deplete CD4+ T cells, mice were i.p. injected with 10mg/kg of anti-CD4 (Bioxcell; ##BE00031) or 10mg/kg isotype control IgG2b (BioXcell #BE0090) at day 3, 5 and 8 after hepatic metastases implantation. .. To deplete CD4+ T cells, mice were i.p. injected with 10mg/kg of anti-CD4 (Bioxcell; ##BE00031) or 10mg/kg isotype control IgG2b (BioXcell #BE0090) at day 3, 5 and 8 after hepatic metastases implantation.

    Article Title: Inhibition of cell surface GRP78 and activated α 2M interaction attenuates kidney fibrosis
    Article Snippet: .. Three separate studies were conducted: (a) treatment with C38 (made in-house) or the isotype control IgG2b (BioXcell, BE0086) antibodies; (b) Fα2M (made in-house) or the isotype control IgG1 (BioXcell, BE0083) antibodies; or (c) P19 or scrambled control peptide (both made in-house). ..

    Article Title: Mac-1 Promotes Neutrophil Extracellular Traps Formation via ERK Phosphorylation in Renal Ischemia-Reperfusion Injury.
    Article Snippet: .. Anti-CD11b antibody (M1/70; BE0007, 100 μg; BioXCell, USA) or isotype control IgG2b (BE0090, BioXCell, USA) was administered intraperitoneally 2 h pre-surgery. ..

    Article Title: Inhibition of cell surface GRP78 and activated α2M interaction attenuates kidney fibrosis.
    Article Snippet: .. Three separate studies were conducted: (a) treatment with C38 (made in-house) or the isotype control IgG2b (BioXcell, BE0086) antibodies; (b) Fα2M (made in-house) or the isotype control IgG1 (BioXcell, BE0083) antibodies; or (c) P19 or scrambled control peptide (both made in-house). ..

    Article Title: Mac-1 Promotes Neutrophil Extracellular Traps Formation via ERK Phosphorylation in Renal Ischemia-Reperfusion Injury
    Article Snippet: .. Anti-CD11b antibody (M1/70; BE0007, 100 μg; BioXCell, USA) or isotype control IgG2b (BE0090, BioXCell, USA) was administered intraperitoneally 2 h pre-surgery. ..

    Article Title: Chemotherapy-driven intestinal dysbiosis and indole-3-propionic acid rewire myelopoiesis to promote a metastasis-refractory state
    Article Snippet: .. To deplete CD4 + T cells, mice were i.p. injected with 10 mg/kg of anti-CD4 (Bioxcell; ##BE0003-1) or 10 mg/kg isotype control IgG2b (BioXcell #BE0090) at day 3, 5 and 8 after hepatic metastases implantation. ..

    Article Title: Overcoming immunotherapy resistance in bladder cancer with a novel antibody-drug conjugate RC48
    Article Snippet: .. Furthermore, to determine whether the killing of tumors by RC48-ADC depends on CD8+ T cells, InVivoPlus anti-mouse CD8α (Cat: BP0117, Bioxcell, USA) (100 μg/each/3 days) or an isotype control IgG2b (Cat: BP0090, Bioxcell, USA) were used to deplete CD8+ T cells in mice based on RC48-ADC treatment. ..

    Article Title: Induction of senescence renders cancer cells highly immunogenic
    Article Snippet: .. For depletion of immune populations, animals received 100 μg of blocking antibodies (anti-CD4 (clone GK1.5, BioxCell), anti-CD8 (clone 2.43, BioxCell), anti-CD11b (clone M1/70, BioxCell)) or isotype control IgG2b (clone LTF-2, BioxCell) via intraperitoneal injection of a total volume of 100 μL on days -1, 3, and 8 of the immunization experiments. ..

    Blocking Assay:

    Article Title: Induction of senescence renders cancer cells highly immunogenic
    Article Snippet: .. For depletion of immune populations, animals received 100 μg of blocking antibodies (anti-CD4 (clone GK1.5, BioxCell), anti-CD8 (clone 2.43, BioxCell), anti-CD11b (clone M1/70, BioxCell)) or isotype control IgG2b (clone LTF-2, BioxCell) via intraperitoneal injection of a total volume of 100 μL on days -1, 3, and 8 of the immunization experiments. ..



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    Image Search Results


    The effects of PD-L1 blockade on airway hyperresponsiveness, inflammatory cell infiltration, and the expression of immunoglobulin in lung tissues of asthmatic mice were investigated. (A) Hyperresponsive changes in the airways of mice detected after stimulation with different concentrations of acetylcholine. (B) Total cells in BALF. (C) Number of eosinophils in BALF. (D–E) The expression levels of OVA-IgE and IgG1 were measured in BALF. PD-L1, Programmed cell death ligand 1. OVA-IgE, Ovalbumin-specific Immunoglobulin E. OVA-IgG1, Ovalbumin-specific Immunoglobulin G1. Data were presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Biomedical Journal

    Article Title: Anti-PD-L1 improves barrier function and reduces CD103 + DC cell accumulation by regulating gut and lung microbiota and its metabolites to alleviate asthma in juvenile mice

    doi: 10.1016/j.bj.2025.100944

    Figure Lengend Snippet: The effects of PD-L1 blockade on airway hyperresponsiveness, inflammatory cell infiltration, and the expression of immunoglobulin in lung tissues of asthmatic mice were investigated. (A) Hyperresponsive changes in the airways of mice detected after stimulation with different concentrations of acetylcholine. (B) Total cells in BALF. (C) Number of eosinophils in BALF. (D–E) The expression levels of OVA-IgE and IgG1 were measured in BALF. PD-L1, Programmed cell death ligand 1. OVA-IgE, Ovalbumin-specific Immunoglobulin E. OVA-IgG1, Ovalbumin-specific Immunoglobulin G1. Data were presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Based on previous [ , , ] studies with appropriate modifications and our previous pre-experimental protocol, the anti-mouse PD-L1 monoclonal antibody (10 μg/g/mouse, BioXcell, 804722F1B, America) and rat IgG2b (Clone: LTF-2) isotype control antibody (10 μg/g/mouse, BioXcell, 767921D1, America) were administered by i.p. injection on days 0, 7, and 14 of sensitization and 21, 28, and 35 of challenge.

    Techniques: Expressing